Partner study description
Collections were taken from multiple sites on the island of Mayotte. Samples were collected as larvae during March-April 2011 in temporary pools by dipping. Sites included Mtsanga Charifou (-12.991, 45.156) and Combani (-12.779, 45.143). Larvae were stored in 80% ethanol prior to DNA extraction. All specimens contributed were An. gambiae (1). Samples were identified as males or females by the sequencing read coverage of the X chromosome using LookSeq (2).
1. F. Santolamazza, A. della Torre, and A. Caccone. Short report: a new polymerase chain reaction-restriction fragment length polymorphism method to identify anopheles arabiensis from an. gambiae and its two molecular forms from degraded dna templates or museum samples. The American journal of tropical medicine and hygiene, 70:604–6, July 2004. https://doi.org/10.4269/ajtmh.2004.70.604
2. H. M. Manske and D. P. Kwiatkowski. Lookseq: a browser-based viewer for deep sequencing data. Genome Res., 19:2125–2132, August 2009. https://doi.org/10.1101/gr.093443.109
Contributors
Gilbert Le Goff (gilbert.legoff@ird.fr) UMR MIVEGEC, Univ. Montpellier, CNRS, IRD, Montpellier, France.
Vincent Robert (vincent.robert@ird.fr) UMR MIVEGEC, Univ. Montpellier, CNRS, IRD, Montpellier, France.
Maryam Kamali Department of Medical Entomology and Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Entomology, Virginia Tech, Blacksburg, Virginia 24061, USA.
Igor Sharakhov (igor@vt.edu) Department of Entomology, Virginia Tech, Blacksburg, Virginia 24061, USA.
Department of Genetics and Cell Biology, Tomsk State University, Tomsk 634050, Russia.