Partner study description
Samples were contributed from collections of indoor resting adults made by spray catch from Monomtenga in central Burkina Faso (12.06, -1.17). These specimens were sorted morphologically to An. gambiae s.l.. Ovaries of half-gravid females were dissected and placed in numbered individual micro-tubes containing modified Carnoy’s solution (1:3 glacial acetic acid: 100% ethanol). Carcasses were placed in correspondingly numbered micro-tubes over desiccant. Genomic DNA was isolated from individual mosquitoes using one of the following: DNeasy Extraction Kit (Qiagen, Valencia, CA), Puregene kit (Gentra Systems, Inc., Minneapolis, MN), DNAzol kit (Molecular Research Center, Inc., Cincinnati, OH.) or Easy-DNA kit (Invitrogen, Carlsbad, CA). An. gambiae s.s. and its molecular forms were identified using one of two rDNA-based PCR/RFLP assays, (1) or (2). Ovaries from specimens of the desired species were subject to polytene chromosome analysis.
1. C. Fanello, F. Santolamazza, and A. della Torre. Simultaneous identification of species and molecular forms of the anopheles gambiae complex by pcr-rflp. Med Vet Entomol, 16:461–464, December 2002. https://doi.org/10.1046/j.1365-2915.2002.00393.x
2. F. Santolamazza, A. della Torre, and A. Caccone. Short report: a new polymerase chain reaction-restriction fragment length polymorphism method to identify anopheles arabiensis from an. gambiae and its two molecular forms from degraded dna templates or museum samples. The American journal of tropical medicine and hygiene, 70:604–6, July 2004. https://doi.org/10.4269/ajtmh.2004.70.604
Contributors
Carlo Costantini (carlo.costantini@ird.fr) UMR MIVEGEC, Univ. Montpellier, CNRS, IRD, Montpellier, France.
N’Fale Sagnon Centre National de Recherche et Formation sur le Paludisme (CNRFP), 01 BP 2208 Ouagadougou 01, Burkina Faso.
Nora J. Besansky (nbesansk@nd.edu) Eck Institute for Global Health & Department of Biological Sciences, University of Notre Dame, IN 46556, USA.