Partner study description
The Target Malaria project contributed samples from collections made in three villages separated by at most 30km: Bana (11.233, -4.472), Souroukoudinga (11.235, -4.535) and Pala (11.150, -4.235). These collections were made in July-August 2012, July and October 2014, and January, February and April 2015. The area is agricultural, with rice-growing areas near Bana and Souroukoudinga, and a large mango grove near Pala. Female mosquitoes were collected by human landing catch, pyrethrum spray collection or aspiration. Males were collected by swarm netting. Both An. gambiae and An. coluzzii (1) were collected. Specimens were stored in 80% ethanol and DNA was extracted using the DNeasy Tissue Kit (Qiagen) or using a simple CTAB method.
Acknowledgments
We would like to thank the technicians of the Institute de Recherche en Sciences de la Santé/Target Malaria Burkina Faso, including Guel Hyacinthe, Diabate Brama, Ilboudo Seni, Kabre Rasmane, Diabate Noufou and Yeye Pascal, for their contributions to sample collections.
1. Elien E. Wilkins, Paul I. Howell, and Mark Q. Benedict. Imp pcr primers detect single nucleotide polymorphisms for anopheles gambiae species identification, mopti and savanna rdna types, and resistance to dieldrin in anopheles arabiensis. Malaria J, 5:125, December 2006. https://doi.org/10.1186/1475-2875-5-125
Contributors
Abdoulaye Diabaté Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, B.P. 545, Burkina Faso.
Patric Stephane Epopa Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, B.P. 545, Burkina Faso.
Franck Yao Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, B.P. 545, Burkina Faso.
Samantha O’Loughlin (s.oloughlin@imperial.ac.uk) Department of Life Sciences, Imperial College, Silwood Park, Ascot, Berkshire SL5 7PY, UK.
Austin Burt Department of Life Sciences, Imperial College, Silwood Park, Ascot, Berkshire SL5 7PY, UK.