Partner study description
Adult mosquitoes were obtained by rearing larvae collected from breeding sites along the main roads connecting the municipalities of Kilamba-Kiaxi and Viana, Luanda province (-8.821, 13.291), in April/May 2009. These are peri-urban areas where malaria reaches hyperendemic levels. All specimens collected in the study area were typed as An. coluzzii (1) although An. melas and An. arabiensis have also been recorded in the province (2, 3). Specimens were stored on silica gel and DNA extraction was performed by a phenol-chloroform protocol described in (4).
See http://hdl.handle.net/10362/11354 for futher details of this study.
1. C. Fanello, F. Santolamazza, and A. della Torre. Simultaneous identification of species and molecular forms of the anopheles gambiae complex by pcr-rflp. Med Vet Entomol, 16:461–464, December 2002. https://doi.org/10.1046/j.1365-2915.2002.00393.x
2. Nelson Cuamba, Kwang Shik Choi, and Harold Townson. Malaria vectors in angola: distribution of species and molecular forms of the Anopheles gambiae complex, their pyrethroid insecticide knockdown resistance (kdr) status and Plasmodium falciparum sporozoite rates. Malaria J, 5:2, January 2006. https://doi.org/10.1186/1475-2875-5-2
3. M. Calzetta, F. Santolamazza, G. C. Carrara, P. J. Cani, F. Fortes, M. A. Di Deco, A. della Torre, and V. Petrarca. Distribution and chromosomal characterization of the Anopheles gambiae complex in angola. The American Journal of Tropical Medicine and Hygiene, 78:169–175, January 2008. https://doi.org/10.4269/ajtmh.2008.78.169
4. M. J. Donnelly, N. Cuamba, J. D. Charlwood, F. H. Collins, and H. Townson. Population structure in the malaria vector, Anopheles arabiensis patton, in east africa. Heredity, 83:408–417, October 1999. https://doi.org/10.1038/sj.hdy.6885930
Contributors
Arlete D. Troco Programa Nacional de Controle da Malária, Direcção Nacional de Saúde Pública, Ministério da Saúde, Luanda, Angola.
João Pinto (jpinto@ihmt.unl.pt) Global Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Rua da Junqueira 100, 1349–008 Lisbon, Portugal.