Partner study description

As a part of clinical studies being undertaken by the KEMRI-Wellcome Trust Research Programme, samples were collected in the Kilifi district of Kenya. Alexis Nzila and his colleagues adapted these field isolates to laboratory culture and tested their in vitro sensitivity to antimalarial drugs including lumefantrine, chloroquine, piperaquine and dihydroartemisinin (DHA). The phenotypic data are now being used in genome-wide association studies with parasite genotypes generated by Illumina sequencing to identify regions associated to decreased sensitivity to the antimalarial drugs.

Publications

• Genomic epidemiology of artemisinin resistant malaria
  MalariaGEN P. falciparum Community Project
  eLife, 2016; 5 e08714
  10.7554/eLife.08714
• Analysis of Plasmodium falciparum diversity in natural infections by deep sequencing
  Manske et al.
  Nature, 2012; 487(7407) 375-9
  10.1038/nature11174
• Population genetic analysis of Plasmodium falciparum parasites using a customized Illumina GoldenGate genotyping assay
  Campino S et al.
  PLoS One, 2011; 6(6) e20251
  10.1371/journal.pone.0020251