Checklist for shipping mosquito samples
We cannot accept any samples or data until the below checklist is complete. If you have any queries regarding any aspect in relation to your partner study, please contact support@malariagen.net, particularly if you require support applying for Nagoya approval and to send your completed sample information sheet.
- The study has a MalariaGEN Study ID
- Relevant ethics approvals are in place and have been shared with MalariaGEN if applicable
- Agreement from National authorities in the country of sample origin in line with the Nagoya Protocol is in place and signed off by Wellcome Sanger Institute where applicable
- Samples meet requirements and sampling/shipping protocols have been followed
- A sample information sheet has been fully completed as per instructions and shared with MalariaGEN
1. Study ID
All MalariaGEN collaborations have a Study ID. We can only accept samples from approved partner studies. This should have been completed during study set-up.
2. Ethics approvals
All relevant ethics approvals for your study should be shared with MalariaGEN prior to receipt and processing of samples in line with good ethical practice. This should have been completed during study set-up, but please provide any updated approvals as they are agreed.
3. Nagoya approval
MalariaGEN cannot accept samples at Wellcome Sanger Institute without relevant Nagoya approval. This should have been completed during study set-up but the in-country process may have changed since and must be checked prior to each sample shipment. Full details can be found on the ABSCH Clearing House, but MalariaGEN are able to help with this process. Material Transfer Agreements are often a requirement, these should use your Institutional template so MalariaGEN does not provide a template as a resource. Wellcome Sanger Institute will need to sign off that due process has been followed.
Please note that approval is required from the country the samples originated in and acquiring approval can be a lengthy process that is best started as soon as possible.
For transfer of data only to MalariaGEN, Nagoya approval is not always required but a data-sharing agreement must be in place with the Wellcome Sanger Institute.
4. Sample requirements
We can only accept whole mosquitoes that have been plated in ethanol. The best sequencing results come from mosquitoes that have been collected straight into ethanol, though previously desiccated mosquitoes that have been transferred into ethanol are also viable. We recommend transfer to ethanol as soon as possible. We may also accept extracted DNA with a minimum volume of 200ng in 100ul.
Species that we routinely process are:
- Anopheles gambiae
- Anopheles funestus
Download the collection shipping protocol
5. Sample Information Sheets (SIS)
The sample information sheet must be emailed to support@malariagen.net before shipping samples or sending data, and a printed copy that must be included within any sample shipment.
Each sample information sheet is an excel file that contains three tabs:
- An ‘Instructions’ tab, providing guidance on how to complete each sample metadata column
- An ‘Anopheles Samples’ tab that must be fully completed for each sample
- A ‘Location’ tab that must be fully completed for all samples